Lt mainly changes bazolateralnoy actin ...

We have found that alternative sigma factors, TetR and BotR, positively regulates the production of tetanus toxin (tent) and botulinum toxin and botulinum, as well as related non-toxic proteins forming complexes BoNT

C. Tetanus

and C. botulism, respectively. BT and tents synthesis is strictly regulated during the transition period between the end of exponential growth and early stationary phase. Clostridial toxins binary (C. perfringens

inch, toxin C.

botulinum toxin C2) is an attractive tool for car heterogeneous proteins in target cells, as well as functional areas are located in two separate proteins. We found that the binding component (B) of little jot toxin forms channels in lipid bilayer and Ib, when associated with the target cell membrane, oligomerizes and then distributed in detergent resistant membranes mikrodomenov allows movement of the enzyme component (Ia) by endosom membrane. Great clostridia toxins, such as LT from C. sordellii

and Ras GTPases. We have described that LT specifically binds to phosphatidylserine (PS), and that LT glucosylation activity above when lining Rac associated with the lipid membrane, mainly enriched PS. LT mainly changes bazolateralnoy actin and E-kadherina intercellular connections polarized epithelial cells, causing apoptosis in myeloid cell lines as well as degeneration and regeneration of skeletal neuromuscular tissue. Expert work on botulism include: preparation and production of recombinant antigens BoNTs, production of specific polyclonal and monoclonal antibodies, genotyping and sequencing botulinum toxin genes C.

Botulinum strains. CNR anaerobic bacteria and botulism involved in identification of anaerobic strains isolated in hospital laboratories strattera prescription, as well as diagnosis and investigation of botulism in France. Taxonomic study of certain groups of anaerobes, and genotyping and characterization of C. difficile strains arise in the process.